plasmids pc0003 Search Results


plasmids pc0003  (ATCC)
93
ATCC plasmids pc0003
Plasmids Pc0003, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmids pc0003/product/ATCC
Average 93 stars, based on 1 article reviews
plasmids pc0003 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
pc0053  (Addgene inc)
93
Addgene inc pc0053
Pc0053, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pc0053/product/Addgene inc
Average 93 stars, based on 1 article reviews
pc0053 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
pspcas13b crrna backbone  (Addgene inc)
95
Addgene inc pspcas13b crrna backbone
Pspcas13b Crrna Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pspcas13b crrna backbone/product/Addgene inc
Average 95 stars, based on 1 article reviews
pspcas13b crrna backbone - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier
plasmids pc0003  (Addgene inc)
92
Addgene inc plasmids pc0003
Plasmids Pc0003, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmids pc0003/product/Addgene inc
Average 92 stars, based on 1 article reviews
plasmids pc0003 - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
non targeting grna plasmid  (Addgene inc)
93
Addgene inc non targeting grna plasmid
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
Non Targeting Grna Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/non targeting grna plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
non targeting grna plasmid - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
103864 pc0053 cmv ie dpspcas13b gs adar2dd e488q delta 984 1090 103869  (Addgene inc)
93
Addgene inc 103864 pc0053 cmv ie dpspcas13b gs adar2dd e488q delta 984 1090 103869
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
103864 Pc0053 Cmv Ie Dpspcas13b Gs Adar2dd E488q Delta 984 1090 103869, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/103864 pc0053 cmv ie dpspcas13b gs adar2dd e488q delta 984 1090 103869/product/Addgene inc
Average 93 stars, based on 1 article reviews
103864 pc0053 cmv ie dpspcas13b gs adar2dd e488q delta 984 1090 103869 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
eicsm6  (Addgene inc)
92
Addgene inc eicsm6
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
Eicsm6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eicsm6/product/Addgene inc
Average 92 stars, based on 1 article reviews
eicsm6 - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
pc0043 crrna  (Addgene inc)
92
Addgene inc pc0043 crrna
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
Pc0043 Crrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pc0043 crrna/product/Addgene inc
Average 92 stars, based on 1 article reviews
pc0043 crrna - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
backbone plasmid pc0043-pspcas13b crrna backbone  (Addgene inc)
90
Addgene inc backbone plasmid pc0043-pspcas13b crrna backbone
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
Backbone Plasmid Pc0043 Pspcas13b Crrna Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/backbone plasmid pc0043-pspcas13b crrna backbone/product/Addgene inc
Average 90 stars, based on 1 article reviews
backbone plasmid pc0043-pspcas13b crrna backbone - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
pc0043  (Addgene inc)
93
Addgene inc pc0043
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
Pc0043, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pc0043/product/Addgene inc
Average 93 stars, based on 1 article reviews
pc0043 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
pc0043 pspcas13b grna non target  (Addgene inc)
93
Addgene inc pc0043 pspcas13b grna non target
(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with <t>gRNA</t> control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.
Pc0043 Pspcas13b Grna Non Target, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pc0043 pspcas13b grna non target/product/Addgene inc
Average 93 stars, based on 1 article reviews
pc0043 pspcas13b grna non target - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
pc0039-cmv-dpspcas13b-gs-adar2dd(e488q) (plasmid #103849)  (Addgene inc)
N/A
   Buy from Supplier

Image Search Results


(A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.

Journal: bioRxiv

Article Title: Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein

doi: 10.1101/614859

Figure Lengend Snippet: (A) Schematic representation of positions of m 6 A site within CYB5A mRNA and the regions targeted by three gRNAs, respectively; (B) m 6 A RIP-qPCR analysis of CYB5A mRNA in HeLa and HEK293T cells; (C) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HeLa and HEK293T cells as compared to their corresponding Mettl3 knocked down cells; (D) The expression of CYB5A in HEK293T cells transfected with wild type Cas13b and gRNAs for 24 h; (E) The threshold cycle (CT) of qPCR showing SELECT results for detecting m 6 A site in CDS region of CYB5A at 48 A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (F) The mRNA expression of CYB5A in HEK293T cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA1/2/3, respectively, for 24 h; (G) HER293T cells were pre-transfected with dCas13b or dCas13b-ALKBH5 with gRNA2 for CYB5A for 24 h and then further further treated with Act-D for the indicated times, and mRNA expression of CYB5A was measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments. ** p<0.01 compared with control.

Article Snippet: The original PspCas13b plasmid (Addgene plasmid #103866), gRNA plasmid (Addgene plasmid #103854) and non-targeting gRNA plasmid (Addgene plasmid #103868) were purchased from Addgene.

Techniques: Expressing, Transfection, Control, Quantitative RT-PCR